Bio203 Assignment No.2 Solution | Bio203 Assignment No.2 solved | BIO203

Bio203 Assignment No.2 Solution | Bio203 Assignment No.2 solved | BIO203

This is Bio203 Assignment No.2 solution which is solved by Indus Stream. This Bio203 Assignment No.2 solved and the first viewer can used it as it has. 

Solution: Bio203 Assignment No.2 Solution

Answer 1: Reverse Transcriptase and Real time PCR is used in the above scenario, where students should amplify and measure(detect) the quantity of DNA as well. It is because Real-Time which also called as Quantitative PCR and abbreviated as (qPCR) is used to measures and amplify the DNA. This kind of PCR is choosed because it is speedy. 

Answer 2: First PCR students should perform is RT-PCR (Reverse Transcriptase PCR), because of the fact, we should first examine the sample. 

Answer 4: Blank PCR is used to either avoid contamination during amplification. 

Question: Bio203 Assignment No.2 

A student has to synthesize complementary DNA (cDNA) from RNA through polymerase chain Reaction (PCR) . Nexr, he has to amplify and detect the quantity of the cDNA. from the following options, he has to select the types of PCR which he should performs to do the above mentioned task. Reverse Transcriptase PCR Long PCR Real time PCR Allele specific PCR a) Explain which of the following types of PCR should the student performs and why? b) which type of PCR should be performed first and why? C) Also , explain why the other types of PCR are not a suitable option. D) the student also performed a blank reaction along with the other sample in the PCR . Explain what blank reaction is and why it is performed?